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MedChemExpress ppar γ expression
Ppar γ Expression, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppar+%CE%B3+expression/pm42045186-80-23-32?v=MedChemExpress
Average 94 stars, based on 22 article reviews

ppar γ expression - by Bioz Stars, 2026-07

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Fig. 1. PPARγ2 and Tnpo1 bind via disulfide bonds. (A–C) Six-week-old male C57BL/6 mice were intravenously injected via the tail vein with 4 × 109 plaque-forming units of mock adenovirus or PPARγ2 adenovirus (adPPARγ2). Livers were dissected 2 weeks later for analysis (n = 3/group). (A) Hepatic TG levels. **P < 0.01 vs. mice infected with mock adenovirus. (B) Immunoprecipitation of PPARγ2/Tnpo1, PPARγ2/importin-α, and PPARγ2/importin-β complexes from liver samples. (C) Immunoprecipitation of PPARγ2/Tnpo1 complex from liver samples with or without 10 mM DTT treatment. (D–F) Immunoprecipitation of PPARγ2:ΔCys (Ser→Cys)/Tnpo1 (D), PPARγ2:WT (Cys→Ser)/ Tnpo1 (E), and PPARγ2/Tnpo1:WT (Cys→Ser) complexes (F). (G) PPARγ2/Tnpo1 complex (yellow), PPARγ2 (green), and Tnpo1 (red) in mouse primary cultured hepatocytes transfected with PPARγ2 or PPARγ2:ΔCys expression vector for 48 h, then treated with 250 μM H2O2 or left untreated for 6 h. Cell lysates were immunoprecipitated with anti-PPARγ antibody, treated with 10 mM DTT or left untreated, and then subjected to non-reducing sodium dodecyl sulphate–polya- crylamide gel electrophoresis followed by western blotting. All data are expressed as mean ± SEM. P value was calculated using unpaired Student's t-test in A. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Free radical biology & medicine

Article Title: Redox-dependent PPARγ/Tnpo1 complex formation enhances PPARγ nuclear localization and signaling.

doi: 10.1016/j.freeradbiomed.2020.06.005

Figure Lengend Snippet: Fig. 1. PPARγ2 and Tnpo1 bind via disulfide bonds. (A–C) Six-week-old male C57BL/6 mice were intravenously injected via the tail vein with 4 × 109 plaque-forming units of mock adenovirus or PPARγ2 adenovirus (adPPARγ2). Livers were dissected 2 weeks later for analysis (n = 3/group). (A) Hepatic TG levels. **P < 0.01 vs. mice infected with mock adenovirus. (B) Immunoprecipitation of PPARγ2/Tnpo1, PPARγ2/importin-α, and PPARγ2/importin-β complexes from liver samples. (C) Immunoprecipitation of PPARγ2/Tnpo1 complex from liver samples with or without 10 mM DTT treatment. (D–F) Immunoprecipitation of PPARγ2:ΔCys (Ser→Cys)/Tnpo1 (D), PPARγ2:WT (Cys→Ser)/ Tnpo1 (E), and PPARγ2/Tnpo1:WT (Cys→Ser) complexes (F). (G) PPARγ2/Tnpo1 complex (yellow), PPARγ2 (green), and Tnpo1 (red) in mouse primary cultured hepatocytes transfected with PPARγ2 or PPARγ2:ΔCys expression vector for 48 h, then treated with 250 μM H2O2 or left untreated for 6 h. Cell lysates were immunoprecipitated with anti-PPARγ antibody, treated with 10 mM DTT or left untreated, and then subjected to non-reducing sodium dodecyl sulphate–polya- crylamide gel electrophoresis followed by western blotting. All data are expressed as mean ± SEM. P value was calculated using unpaired Student's t-test in A. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mutant mouse Tnpo1 and PPARγ2 expression vectors were prepared using the KOD-Plus-Mutagenesis kit (Toyobo, Osaka, Japan) from MycDDK-tagged mouse Tnpo1 and PPARγ2 expression vectors (Origene).

Techniques: Injection, Infection, Immunoprecipitation, Cell Culture, Transfection, Expressing, Plasmid Preparation, Nucleic Acid Electrophoresis, Western Blot

Fig. 3. Cytosolic H2O2/Tnpo1-dependent nuclear translocation of PPARγ2 enhances PPARγ2 target gene expression and TG accumulation in hepatocytes. (A–C) WT and Sod1-deficient hepatocytes were infected with mock adenovirus or adPPARγ2 at a MOI of 50 for 48 h, followed by culturing with or without 5 μM DMNQ for 6 h. (D–F) WT hepatocytes were infected with mock adenovirus or adPPARγ2 at an MOI of 50, transfected with Tnpo1 or control siRNA for 24 h, and cultured with or without 5 μM DMNQ for 6 h. (A, D) (Left) Oil Red O staining. (Right) Quantification of Oil Red O staining. (B, C, E, F) Quantification of Pparg2, Fsp27, Fabp1, and Fabp4 mRNA levels (B, E) and the amount of DNA-bound PPARγ (C, F) in WT and Sod1-deficient hepatocytes (n = 5/group). *P < 0.05, **P < 0.01 vs. WT hepatocytes infected with mock adenovirus without DMNQ treatment (A–C) and vs. hepatocytes infected with mock adenovirus, transfected with control siRNA, and without DMNQ treatment (D–F). All data are expressed as mean ± SEM. P value was calculated by one-way ANOVA in A, B, C, D, E, and F. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Free radical biology & medicine

Article Title: Redox-dependent PPARγ/Tnpo1 complex formation enhances PPARγ nuclear localization and signaling.

doi: 10.1016/j.freeradbiomed.2020.06.005

Figure Lengend Snippet: Fig. 3. Cytosolic H2O2/Tnpo1-dependent nuclear translocation of PPARγ2 enhances PPARγ2 target gene expression and TG accumulation in hepatocytes. (A–C) WT and Sod1-deficient hepatocytes were infected with mock adenovirus or adPPARγ2 at a MOI of 50 for 48 h, followed by culturing with or without 5 μM DMNQ for 6 h. (D–F) WT hepatocytes were infected with mock adenovirus or adPPARγ2 at an MOI of 50, transfected with Tnpo1 or control siRNA for 24 h, and cultured with or without 5 μM DMNQ for 6 h. (A, D) (Left) Oil Red O staining. (Right) Quantification of Oil Red O staining. (B, C, E, F) Quantification of Pparg2, Fsp27, Fabp1, and Fabp4 mRNA levels (B, E) and the amount of DNA-bound PPARγ (C, F) in WT and Sod1-deficient hepatocytes (n = 5/group). *P < 0.05, **P < 0.01 vs. WT hepatocytes infected with mock adenovirus without DMNQ treatment (A–C) and vs. hepatocytes infected with mock adenovirus, transfected with control siRNA, and without DMNQ treatment (D–F). All data are expressed as mean ± SEM. P value was calculated by one-way ANOVA in A, B, C, D, E, and F. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques: Translocation Assay, Targeted Gene Expression, Infection, Transfection, Control, Cell Culture, Staining

Fig. 4. Cytosolic H2O2/Tnpo1-dependent nuclear translocation of PPARγ2 promotes hepatic TG accumulation in mice infected with adPPARγ2. (A–F) Six-week-old male C57BL/6 WT and Sod1-deficient mice were intravenously injected via the tail vein with 4 × 109 plaque-forming units of mock adenovirus and PPARγ2 and SOD1 adenoviruses (adPPARγ2 and adSOD1, respectively) in various combinations. Livers were dissected from mice 2 weeks later for analysis. (G–J) Six-week-old male C57BL/6 WT and Sod1-deficient mice were intravenously injected via the tail vein with 4 × 109 plaque-forming units of mock adenovirus or adPPARγ2, followed by injection of control or Tnpo1 siRNA (1 mg/kg body weight) after 2 days. Livers were dissected from mice 12 days later for analysis. (A, G) Representative H&E-stained liver sections. (F) Immunoprecipitation of PPARγ2/Tnpo1 complex from liver tissue. (B–E, H–J) Quantification of hepatic TG (B, H), cytosolic H2O2 (C), and Pparg2, Fsp27, Fabp1, and Fabp4 mRNA (D, I) levels and the amount of DNA-bound PPARγ (E, J) (n = 5/group). **P < 0.01 vs. WT mice infected with mock adenovirus (B–E); and *P < 0.05, **P < 0.01 vs. WT mice infected with mock adenovirus and treated with control siRNA (H–J). All data are expressed as mean ± SEM. P value was calculated by one-way ANOVA in B, C, D, E, H, I, and J.

Journal: Free radical biology & medicine

Article Title: Redox-dependent PPARγ/Tnpo1 complex formation enhances PPARγ nuclear localization and signaling.

doi: 10.1016/j.freeradbiomed.2020.06.005

Figure Lengend Snippet: Fig. 4. Cytosolic H2O2/Tnpo1-dependent nuclear translocation of PPARγ2 promotes hepatic TG accumulation in mice infected with adPPARγ2. (A–F) Six-week-old male C57BL/6 WT and Sod1-deficient mice were intravenously injected via the tail vein with 4 × 109 plaque-forming units of mock adenovirus and PPARγ2 and SOD1 adenoviruses (adPPARγ2 and adSOD1, respectively) in various combinations. Livers were dissected from mice 2 weeks later for analysis. (G–J) Six-week-old male C57BL/6 WT and Sod1-deficient mice were intravenously injected via the tail vein with 4 × 109 plaque-forming units of mock adenovirus or adPPARγ2, followed by injection of control or Tnpo1 siRNA (1 mg/kg body weight) after 2 days. Livers were dissected from mice 12 days later for analysis. (A, G) Representative H&E-stained liver sections. (F) Immunoprecipitation of PPARγ2/Tnpo1 complex from liver tissue. (B–E, H–J) Quantification of hepatic TG (B, H), cytosolic H2O2 (C), and Pparg2, Fsp27, Fabp1, and Fabp4 mRNA (D, I) levels and the amount of DNA-bound PPARγ (E, J) (n = 5/group). **P < 0.01 vs. WT mice infected with mock adenovirus (B–E); and *P < 0.05, **P < 0.01 vs. WT mice infected with mock adenovirus and treated with control siRNA (H–J). All data are expressed as mean ± SEM. P value was calculated by one-way ANOVA in B, C, D, E, H, I, and J.

Techniques: Translocation Assay, Infection, Injection, Control, Staining, Immunoprecipitation

Fig. 5. Cytosolic H2O2/Tnpo1-dependent nuclear translocation of PPARγ2 promotes hepatic TG accumulation in mice fed an HFD. (A–F) Six-week-old male C57BL/6 mice were fed a control diet or HFD for 4 weeks, then intravenously injected via the tail vein with control, Tnpo1, Sod1, or Pparg2 siRNA (1 mg/kg body weight) in various combinations. Livers were dissected from the mice 10 days later for analysis (n = 5/group). (A) Representative H&E-stained liver sections. (B–D) Quantification of TG (B), cytosolic H2O2 (C), and Pparg2, Fsp27, Fabp1, and Fabp4 mRNA levels (D) in the liver (n = 5/group). **P < 0.01 vs. mice fed a control diet and treated with control siRNA. (E) Immunoprecipitation of PPARγ2/Tnpo1 complex in the liver. (F) Quantification of the amount of DNA- bound PPARγ in the liver (n = 5/group). **P < 0.01 vs. mice fed a control diet and treated with control siRNA. #P < 0.05, ##P < 0.01 vs. mice fed an HFD diet and treated with control siRNA. All data are expressed as mean ± SEM. P value was calculated by one-way ANOVA in B, C, D, E, and F.

Journal: Free radical biology & medicine

Article Title: Redox-dependent PPARγ/Tnpo1 complex formation enhances PPARγ nuclear localization and signaling.

doi: 10.1016/j.freeradbiomed.2020.06.005

Figure Lengend Snippet: Fig. 5. Cytosolic H2O2/Tnpo1-dependent nuclear translocation of PPARγ2 promotes hepatic TG accumulation in mice fed an HFD. (A–F) Six-week-old male C57BL/6 mice were fed a control diet or HFD for 4 weeks, then intravenously injected via the tail vein with control, Tnpo1, Sod1, or Pparg2 siRNA (1 mg/kg body weight) in various combinations. Livers were dissected from the mice 10 days later for analysis (n = 5/group). (A) Representative H&E-stained liver sections. (B–D) Quantification of TG (B), cytosolic H2O2 (C), and Pparg2, Fsp27, Fabp1, and Fabp4 mRNA levels (D) in the liver (n = 5/group). **P < 0.01 vs. mice fed a control diet and treated with control siRNA. (E) Immunoprecipitation of PPARγ2/Tnpo1 complex in the liver. (F) Quantification of the amount of DNA- bound PPARγ in the liver (n = 5/group). **P < 0.01 vs. mice fed a control diet and treated with control siRNA. #P < 0.05, ##P < 0.01 vs. mice fed an HFD diet and treated with control siRNA. All data are expressed as mean ± SEM. P value was calculated by one-way ANOVA in B, C, D, E, and F.

Techniques: Translocation Assay, Control, Injection, Staining, Immunoprecipitation

Western blot analysis for the overexpression of PPARG in A549 cell line. (A) Cell lysates from different time point post PPARG transfection were run on SDS PAGE and probed with mAb for PPARG. The blots were developed using chemiluminescence. (B) Overexpression of PPARG (TT-transient transfection) was evaluated in irradiated (5 Gy) and non-irradiated conditions in A549 cells. (C) Detailed plan used for experiments to assess the effect of PPARG overexpression on A549 cell line in combination with radiation.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Western blot analysis for the overexpression of PPARG in A549 cell line. (A) Cell lysates from different time point post PPARG transfection were run on SDS PAGE and probed with mAb for PPARG. The blots were developed using chemiluminescence. (B) Overexpression of PPARG (TT-transient transfection) was evaluated in irradiated (5 Gy) and non-irradiated conditions in A549 cells. (C) Detailed plan used for experiments to assess the effect of PPARG overexpression on A549 cell line in combination with radiation.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Western Blot, Over Expression, Transfection, SDS Page, Irradiation

PPARG radiosensitize A549 cells. A549 cells were transfected with PPARG and irradiated 24 h post-transfection and processed 48 h after transfection. The groups included CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT). The cells were processed for various assays. (A) Morphological analysis was done 24 h post-radiation (48 h post PPARG transfection). (B) Cell count was determined using hemocytometer 24 h post-radiation. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (C) cell viability was assessed by MTT assay 24 h post-radiation. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times. (D) Cell viability was assessed by SRB (Sulforhodamine) assay 24 h post-radiation. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3).

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: PPARG radiosensitize A549 cells. A549 cells were transfected with PPARG and irradiated 24 h post-transfection and processed 48 h after transfection. The groups included CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT). The cells were processed for various assays. (A) Morphological analysis was done 24 h post-radiation (48 h post PPARG transfection). (B) Cell count was determined using hemocytometer 24 h post-radiation. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (C) cell viability was assessed by MTT assay 24 h post-radiation. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times. (D) Cell viability was assessed by SRB (Sulforhodamine) assay 24 h post-radiation. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3).

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Transfection, Irradiation, Control, Cell Counting, Standard Deviation, MTT Assay

Cell death induced by PPARG is apoptotic-c in nature. A549 cells were transfected with PPARG (PPARG (TT) and irradiated 24 h post-transfection and processed 48 h after transfection. These samples which includes CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for various assays. (A) Sub G1 phase analysis was performed using flow cytometry, 48 h post-transfection. The plot on the right shows quantification of the same. (B) PI uptake was performed 48 h post-transfection, and the quantification has been shown on the right. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times. (C) Annexin PI (Propidium iodide) was performed to evaluate apoptosis, 48 h post-transfection. FL1-H represents the filter for FITC whereas FL2-H represents filter for PI. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (D) Gamma H2AX foci were measured 1 h post-irradiation in A549 cells and average foci/cell was calculated.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Cell death induced by PPARG is apoptotic-c in nature. A549 cells were transfected with PPARG (PPARG (TT) and irradiated 24 h post-transfection and processed 48 h after transfection. These samples which includes CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for various assays. (A) Sub G1 phase analysis was performed using flow cytometry, 48 h post-transfection. The plot on the right shows quantification of the same. (B) PI uptake was performed 48 h post-transfection, and the quantification has been shown on the right. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times. (C) Annexin PI (Propidium iodide) was performed to evaluate apoptosis, 48 h post-transfection. FL1-H represents the filter for FITC whereas FL2-H represents filter for PI. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (D) Gamma H2AX foci were measured 1 h post-irradiation in A549 cells and average foci/cell was calculated.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Transfection, Irradiation, Control, Flow Cytometry, Standard Deviation, Quantitation Assay

Death induced by PPARG in combination with radiation is BAX mediated. A549 cells were transfected with PPARG (PPARG (TT) and irradiated 24 h post-transfection, and processed 48 h after transfection. These samples which include CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for Western blotting (A) The effect of PPARG overexpression on the levels of BAX and cytocrome c was evaluated using Western blotting. GAPDH was used as a loading control. The graph represents the relative quantification of BAX. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 (B) The effect of PPARG overexpression on the levels of BCL2 was evaluated using Western blotting. ACTB was used as a loading control. The graph represents the relative quantification of BCL2. The protein level has been normalized with the loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times. (C) The effect of PPARG overexpression on the levels of cleaved PARP, 48 h post-transfection was evaluated using Western blotting. GAPDH was used as a loading control. The graph represents the relative quantification of cleaved PARP levels where the protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Death induced by PPARG in combination with radiation is BAX mediated. A549 cells were transfected with PPARG (PPARG (TT) and irradiated 24 h post-transfection, and processed 48 h after transfection. These samples which include CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for Western blotting (A) The effect of PPARG overexpression on the levels of BAX and cytocrome c was evaluated using Western blotting. GAPDH was used as a loading control. The graph represents the relative quantification of BAX. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 (B) The effect of PPARG overexpression on the levels of BCL2 was evaluated using Western blotting. ACTB was used as a loading control. The graph represents the relative quantification of BCL2. The protein level has been normalized with the loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times. (C) The effect of PPARG overexpression on the levels of cleaved PARP, 48 h post-transfection was evaluated using Western blotting. GAPDH was used as a loading control. The graph represents the relative quantification of cleaved PARP levels where the protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Transfection, Irradiation, Control, Western Blot, Over Expression, Quantitative Proteomics, Standard Deviation

PPARG mediated apoptosis is inversely related to TP53 pathway. A549 cells were transfected with PPARG (PPARG (TT) and irradiated 24 h post-transfection, and processed 48 h after transfection. These samples which include CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for Western blotting. (A) The effect of PPARG overexpression on the levels of TP53 was evaluated using Western blotting. Same GAPDH was used as a loading control as that for PPARG blot in . The graph on the right represents the relative quantification of TP53. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times. (B) The effect of PPARG overexpression on the levels of CDKN1A, the downstream target of TP53 was evaluated using Western blotting. GAPDH was used as a loading control. The graph represents the relative quantification of CDKN1A. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times. (C) . The effect of PPARG overexpression on the levels of CDKN1B was evaluated using Western blotting. GAPDH was used as a loading control. The graph on the right represents the relative quantification of CDKN1B. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: PPARG mediated apoptosis is inversely related to TP53 pathway. A549 cells were transfected with PPARG (PPARG (TT) and irradiated 24 h post-transfection, and processed 48 h after transfection. These samples which include CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for Western blotting. (A) The effect of PPARG overexpression on the levels of TP53 was evaluated using Western blotting. Same GAPDH was used as a loading control as that for PPARG blot in . The graph on the right represents the relative quantification of TP53. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times. (B) The effect of PPARG overexpression on the levels of CDKN1A, the downstream target of TP53 was evaluated using Western blotting. GAPDH was used as a loading control. The graph represents the relative quantification of CDKN1A. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times. (C) . The effect of PPARG overexpression on the levels of CDKN1B was evaluated using Western blotting. GAPDH was used as a loading control. The graph on the right represents the relative quantification of CDKN1B. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The blots were repeated three times.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Transfection, Irradiation, Control, Western Blot, Over Expression, Quantitative Proteomics, Standard Deviation

Similar to its overexpression, PPARG ligand rosiglitazone (RSZ) also induce radiosensitization. A549 cells were treated with agonist of PPARG rosiglitazone (RSZ). The samples which included CONTROL, RADIATION (5 Gy), RSZ, and RAD+RSZ were processed for various assays (A) PPARG translocate to nucleus partially at 24 h and fully after 48 h of treatment with rosiglitazone as indicated by immunofluorescence (B) SF-2 value was calculated on treatment of A549 cells at different doses of radiation along with the treatment of cells with rosiglitazone for 48 h. The experient was performed in triplicate. (C) viability of A549 cell line was assessed 24 h post-radiation with continuous treatment with rosiglitazone for 48 h in A549 cell line. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. (D–F) The effect of PPARG ligand on the levels of TP53 and its downstream target CDKN1A and CDKN1B was evaluated using Western blotting. GAPDH was used as a loading control which is same for CDKN1A and CDKN1B. The graph on the right represents the relative quantification of the blots. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05. The blots were repeated two times. (G) The effect of PPARG ligand on the levels of BAX was evaluated using Western blotting. GAPDH was used as a loading control.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Similar to its overexpression, PPARG ligand rosiglitazone (RSZ) also induce radiosensitization. A549 cells were treated with agonist of PPARG rosiglitazone (RSZ). The samples which included CONTROL, RADIATION (5 Gy), RSZ, and RAD+RSZ were processed for various assays (A) PPARG translocate to nucleus partially at 24 h and fully after 48 h of treatment with rosiglitazone as indicated by immunofluorescence (B) SF-2 value was calculated on treatment of A549 cells at different doses of radiation along with the treatment of cells with rosiglitazone for 48 h. The experient was performed in triplicate. (C) viability of A549 cell line was assessed 24 h post-radiation with continuous treatment with rosiglitazone for 48 h in A549 cell line. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. (D–F) The effect of PPARG ligand on the levels of TP53 and its downstream target CDKN1A and CDKN1B was evaluated using Western blotting. GAPDH was used as a loading control which is same for CDKN1A and CDKN1B. The graph on the right represents the relative quantification of the blots. The protein level has been normalized with loading control. The error bar represents standard deviation in which ∗ P < 0.05. The blots were repeated two times. (G) The effect of PPARG ligand on the levels of BAX was evaluated using Western blotting. GAPDH was used as a loading control.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Over Expression, Control, Immunofluorescence, Standard Deviation, Western Blot, Quantitative Proteomics

PPARG induces cell death in TP53 null cell line. H1299 cells were transfected with PPARG and irradiated 24 h post-transfection and processed 48 h after transfection. These samples which included CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for various assays. (A) Cell survival was studied 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (B) metabolic viability was determined by MTT 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (C) Cell viability was determined by SRB 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3). (D) PI uptake was performed 48 h post-transfection, and the quantification has been shown on the right. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated two times (E) Annexin PI was performed to evaluate apoptosis, 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: PPARG induces cell death in TP53 null cell line. H1299 cells were transfected with PPARG and irradiated 24 h post-transfection and processed 48 h after transfection. These samples which included CONTROL, RAD CONTROL (5 Gy), PPARG (TT), and RAD+PPARG (TT) were processed for various assays. (A) Cell survival was studied 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (B) metabolic viability was determined by MTT 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times (C) Cell viability was determined by SRB 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3). (D) PI uptake was performed 48 h post-transfection, and the quantification has been shown on the right. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated two times (E) Annexin PI was performed to evaluate apoptosis, 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) and repeated three times.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Transfection, Irradiation, Control, Standard Deviation, Quantitation Assay

Cell death induced by PPARG+radiation is more pronounced in H1299 as compared to A549. (A) Comparative analysis of cell death between TP 53 null cell line and A549 was done 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 (B) Comparative analysis of PI uptake was performed 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 and (C) Comparative analysis for Annexin PI was performed to evaluate apoptosis, 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Cell death induced by PPARG+radiation is more pronounced in H1299 as compared to A549. (A) Comparative analysis of cell death between TP 53 null cell line and A549 was done 48 h post-transfection. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 (B) Comparative analysis of PI uptake was performed 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 and (C) Comparative analysis for Annexin PI was performed to evaluate apoptosis, 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Transfection, Standard Deviation, Quantitation Assay

Apoptosis induction in A549 cell line is PPARG dependent. (A) Knockdown of PPARG was done using siRNA specific to PPARG. Effect of PPARG knockdown on A549 cells was determined by various assays. PPARG was co-transfected along with PPARG siRNA and the complete knockdown of PPARG was evaluated using Western blotting. The protein level has been normalized with loading control. (B) Cell survival was studied 48 h post-transfection The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) two times (C) Metabolic viability was determined 48 h post-transfection using MTT assay. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) two times (D) Annexin PI was performed to evaluate apoptosis, 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3).

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Apoptosis induction in A549 cell line is PPARG dependent. (A) Knockdown of PPARG was done using siRNA specific to PPARG. Effect of PPARG knockdown on A549 cells was determined by various assays. PPARG was co-transfected along with PPARG siRNA and the complete knockdown of PPARG was evaluated using Western blotting. The protein level has been normalized with loading control. (B) Cell survival was studied 48 h post-transfection The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) two times (C) Metabolic viability was determined 48 h post-transfection using MTT assay. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3) two times (D) Annexin PI was performed to evaluate apoptosis, 48 h post-transfection. The quantitation has been shown in column graph. The error bar represents standard deviation in which ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. The experiment was done in triplicate ( n = 3).

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Knockdown, Transfection, Western Blot, Control, Standard Deviation, MTT Assay, Quantitation Assay

Inhibition of the survival pathways by PPARG in A549 cells. A549 cells were transfected with PPARG and irradiated 24 h post-transfection, and processed 48 h after transfection. (A) The effect of PPARG overexpression on the levels of total AKT and phospho AKT, 48 h post-transfection was evaluated using Western blotting. ACTB (beta actin) was used as a loading control. (B) The effect of PPARG overexpression on the levels of total P44/42 (MAPK) and phospho P44/42 (MAPK), 48 h post-transfection was evaluated using Western blotting. ACTB (beta actin) was used as a loading control.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Inhibition of the survival pathways by PPARG in A549 cells. A549 cells were transfected with PPARG and irradiated 24 h post-transfection, and processed 48 h after transfection. (A) The effect of PPARG overexpression on the levels of total AKT and phospho AKT, 48 h post-transfection was evaluated using Western blotting. ACTB (beta actin) was used as a loading control. (B) The effect of PPARG overexpression on the levels of total P44/42 (MAPK) and phospho P44/42 (MAPK), 48 h post-transfection was evaluated using Western blotting. ACTB (beta actin) was used as a loading control.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Inhibition, Transfection, Irradiation, Over Expression, Western Blot, Control

Proposed model. The model illustrates our study. In the left panel, the effect of radiation on A549 cells has been shown. As observed in our data, a very less cell death is represented. Right panel shows that the combination of either PPARG agonist or its overexpression, with radiation induces significant amount of radiosensitization leading to enhanced apoptosis in NSCLC.

Journal: Frontiers in Genetics

Article Title: Peroxisome Proliferator Activated Receptor Gamma Sensitizes Non-small Cell Lung Carcinoma to Gamma Irradiation Induced Apoptosis

doi: 10.3389/fgene.2019.00554

Figure Lengend Snippet: Proposed model. The model illustrates our study. In the left panel, the effect of radiation on A549 cells has been shown. As observed in our data, a very less cell death is represented. Right panel shows that the combination of either PPARG agonist or its overexpression, with radiation induces significant amount of radiosensitization leading to enhanced apoptosis in NSCLC.

Article Snippet: A549 cells were transiently transfected with PPARG gene expression plasmid (Addgene# 8895).

Techniques: Over Expression

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